Photochemical synthesis of vitamin D3 (cholecalciferol, D3) occurs cutaneously where pro-vitamin D3 (7-dehydrocholesterol) is converted to pre-vitamin D3 (pre-D3) in response to ultraviolet B (sunlight) exposure. DHCR7 encodes the enzyme 7-dehydrocholesterol (7-DHC) reductase, which converts 7-DHC to cholesterol, thereby removing the substrate from the synthetic pathway of vitamin D3, a precursor of 25-hydroxyvitamin D3.The finding that common variants at DHCR7 are strongly associated with circulating 25-hydroxyvitamin D concentrations suggests that this enzyme could have a larger role in regulation of vitamin D status than has previously been recognised. Vitamin D3, obtained from the isomerization of pre-vitamin D3 in the epidermal basal layers or intestinal absorption of natural and fortified foods and supplements, binds to vitamin D-binding protein (DBP) in the bloodstream, and is transported to the liver. D3 is hydroxylated by liver 25-hydroxylases (25-OHase). The resultant 25-hydroxycholecalciferol (25(OH)D3) is 1-hydroxylated in the kidney by 25-hydroxyvitamin D3-1 -hydroxylase (1-OHase). This yields the active secosteroid 1 ,25(OH)2D3 (calcitriol), which has different effects on various target tissues. The synthesis of 1,25(OH)2D3 from 25(OH)D3 is stimulated by parathyroid hormone (PTH) and suppressed by Ca2+, Pi and 1,25(OH)2D3 itself. The rate-limiting step in catabolism is the degradation of 25(OH)D3 and 1,25(OH)2D3 to 24,25(OH)D3 and 1,24,25(OH)2D3, respectively,which occurs through 24-hydroxylation by 25-hydroxyvitamin D 24-hydroxylase (24-OHase), encoded by the CYP24A1 gene. 24,25(OH)D3 and 1,24,25(OH)2D3 are consequently excreted. Vitamin D activity is mediated through binding of 1,25(OH)2D3 to the vitamin D receptor (VDR), which can regulate transcription of other genes involved in cell regulation, growth, and immunity. VDR modulates the expression of genes by forming a heterodimer complex with retinoid-X-receptors (RXR).

Proteins on this pathway have targeted assays available via the [https://assays.cancer.gov/available_assays?wp_id=WP1531 CPTAC Assay Portal].
Metabolic Process

[INHIBITION:f41a2]	Invalid interaction type.
[STIMULATION:id402e7170]	Invalid interaction type.
[MODIFICATION:id879db83f]	Invalid interaction type.
[STIMULATION:idba7e735a]	Invalid interaction type.
[STIMULATION:id646fbe27]	Invalid interaction type.
[BINDING:ac27b]	Invalid interaction type as an input to reaction.
[Label:cbc34]	 Label cannot be part of reaction. Tranforming to Unknown
[Label:f2831]	 Label cannot be part of reaction. Tranforming to Unknown
[VDR targets:c7c40]	 Shape can not be part of reaction. Tranforming to Unknown
[HeterodimerAssociationReaction:id3edf95c8]	Reaction should contain at least 2 reactants. GraphId: id3edf95c8
[PositiveInfluenceReaction:id879db83f]	Reaction should contain only one reactant and one product.