SARS-CoV-2-Queries

[ en ja nl es pt ]

The Human

Interacting genes and proteins

Understanding the biological processes by which the CoV virion works, requires knowing which human genes and proteins are involved. This is towards the next section of course, but we may first want to know what literature has to say here (annotation is still limited):

SPARQL sparql/humanInteractionCounts.rq (run, edit)

SELECT ?virus ?virusLabel ?gene ?geneLabel ?count WITH {
  SELECT ?virus ?gene (COUNT(DISTINCT ?work) AS ?count) WHERE {
    ?virus wdt:P171+ wd:Q57751738 .
    ?work wdt:P921 ?virus, ?gene .
    ?gene wdt:P703 wd:Q15978631 .
    { ?gene wdt:P31 wd:Q7187 } UNION { ?gene wdt:P31 wd:Q8054 }
  } GROUP BY ?virus ?gene
} AS %ARTICLES WHERE {
  INCLUDE %ARTICLES
  SERVICE wikibase:label { bd:serviceParam wikibase:language "en,en". }
}
ORDER BY DESC(?count) ?virus ?gene

This gives us these numbers of papers:

virus gene count
SARS-CoV-2 (edit) angiotensin I converting enzyme 2 (edit) 24
SARS-CoV-2 (edit) Transmembrane serine protease 2 (edit) 9
SARS-CoV-1 (edit) angiotensin I converting enzyme 2 (edit) 7
SARS-CoV-1 (edit) Transmembrane serine protease 2 (edit) 4
SARSr-CoV (edit) angiotensin I converting enzyme 2 (edit) 3
SARS-CoV-2 (edit) Furin, paired basic amino acid cleaving enzyme (edit) 3
Betacoronavirus (edit) angiotensin I converting enzyme 2 (edit) 2
Middle East respiratory syndrome coronavirus (edit) Dipeptidyl peptidase 4 (edit) 2
SARS-CoV-2 (edit) ACE2 (edit) 2
SARS-CoV-2 (edit) TMPRSS2 (edit) 2
Betacoronavirus (edit) C-reactive protein (edit) 1
Human coronavirus 229E (edit) TMPRSS2 (edit) 1
Human coronavirus 229E (edit) Transmembrane serine protease 2 (edit) 1
Human coronavirus 229E (edit) peptidylprolyl isomerase A (edit) 1
Human coronavirus 229E (edit) Alanyl aminopeptidase, membrane (edit) 1
Human coronavirus 229E (edit) angiotensin I converting enzyme 2 (edit) 1
SARSr-CoV (edit) MASP2 (edit) 1
SARSr-CoV (edit) TNF (edit) 1
SARSr-CoV (edit) Interferon induced transmembrane protein 3 (edit) 1
SARSr-CoV (edit) Interferon induced transmembrane protein 2 (edit) 1
SARSr-CoV (edit) Interferon induced transmembrane protein 1 (edit) 1
Middle East respiratory syndrome coronavirus (edit) Transmembrane serine protease 2 (edit) 1
SARS-CoV-2 (edit) IL6 (edit) 1
SARS-CoV-2 (edit) HLA-E (edit) 1
SARS-CoV-2 (edit) BSG (edit) 1
SARS-CoV-2 (edit) FURIN (edit) 1
SARS-CoV-2 (edit) MX1 (edit) 1
SARS-CoV-2 (edit) IGHV3-53 (edit) 1
SARS-CoV-2 (edit) TRBV11-2 (edit) 1
SARS-CoV-2 (edit) PIKFYVE (edit) 1
SARS-CoV-2 (edit) thioredoxin reductase 1 (edit) 1
SARS-CoV-2 (edit) CCHC-type zinc finger nucleic acid binding protein (edit) 1
SARS-CoV-2 (edit) transmembrane serine protease 4 (edit) 1
SARS-CoV-2 (edit) Transmembrane protein 41B (edit) 1
SARS-CoV-2 (edit) Translocase of outer mitochondrial membrane 70 (edit) 1
SARS-CoV-2 (edit) Von Willebrand factor (edit) 1
SARS-CoV-2 (edit) ADAM metallopeptidase with thrombospondin type 1 motif 13 (edit) 1
SARS-CoV-2 (edit) heme oxygenase 1 (edit) 1
human Coronavirus NL63 (edit) angiotensin I converting enzyme 2 (edit) 1
SARS-CoV-1 (edit) MASP2 (edit) 1
SARS-CoV-1 (edit) TNF (edit) 1
SARS-CoV-1 (edit) C-type lectin domain family 4 member G (edit) 1
SARS-CoV-1 (edit) C-type lectin domain family 4 member M (edit) 1
SARS-CoV-1 (edit) Hepsin (edit) 1
SARS-CoV-1 (edit) Transmembrane serine protease 11D (edit) 1
SARS-CoV-1 (edit) Interferon induced transmembrane protein 3 (edit) 1
SARS-CoV-1 (edit) Interferon induced transmembrane protein 2 (edit) 1
SARS-CoV-1 (edit) Interferon induced transmembrane protein 1 (edit) 1
SARS-CoV-1 (edit) BCL2 like 1 (edit) 1
SARS-CoV-1 (edit) ADAM metallopeptidase domain 17 (edit) 1
SARS-CoV-1 (edit) BCL2 associated X, apoptosis regulator (edit) 1

If you want to see the specific papers (a growing list), use this query:

SPARQL sparql/humanInteractions.rq (run, edit)

SELECT ?date ?virus ?virusLabel ?gene ?geneLabel ?work ?workLabel ?doi WITH {
  SELECT (MAX(?dates) as ?date) ?virus ?gene ?work WHERE {
    ?virus wdt:P171+ wd:Q57751738 .
    ?work wdt:P921 ?virus, ?gene .  
    OPTIONAL { ?work wdt:P577 ?dates . }
    ?gene wdt:P703 wd:Q15978631 .   
    { ?gene wdt:P31 wd:Q7187 } UNION { ?gene wdt:P31 wd:Q8054 }
  } GROUP BY ?virus ?gene ?work
} AS %ARTICLES WHERE {
  INCLUDE %ARTICLES
  OPTIONAL { ?work wdt:P356 ?doi . }
  SERVICE wikibase:label { bd:serviceParam wikibase:language "en,en". }
}
ORDER BY DESC(?date) ?doi ?virus ?gene

Biological processes

WikiPathways [1] is one of the projects involved in the international #covidpathways COVID-19 curation effort and the WikiPathways contributions can be found on this portal.

Reactome [2] and WikiPathways pathways are indexed in Wikidata, and we can query for pathways that have HCoV genes and proteins in their pathways:

SPARQL sparql/pathways.rq (run, edit)

SELECT ?virus ?virusLabel ?thing ?thingLabel ?pathway ?pathwayLabel WHERE {
  VALUES ?virus {
    wd:Q82069695 # SARS-CoV-2
    wd:Q16983360 # HKU1
    wd:Q16991954 # OC43
    wd:Q8351095  # NL63 
    wd:Q16983356 # 229E
    wd:Q4902157  # MERS-CoV
    wd:Q278567   # SARS-CoV
  }
  ?thing wdt:P703 ?virus .
  ?pathway wdt:P31 wd:Q4915012 ; wdt:P527 ?thing .
  SERVICE wikibase:label { bd:serviceParam wikibase:language "en,en". }
} ORDER BY ASC(?virus) ASC(?thing) ASC(?pathway)

This currently lists the following pathways, but with the active curation, it is expected to grow quickly too:

virus thing pathway
SARS-CoV-2 (edit) ORF9c protein [SARS CoV-2] (edit) Mitochondrial immune response to SARS-CoV-2 (edit)
SARS-CoV-2 (edit) S1 Subunit of Spike Protein (edit) Mitochondrial immune response to SARS-CoV-2 (edit)
SARS-CoV-2 (edit) S1 Subunit of Spike Protein (edit) SARS-CoV-2 innate immunity evasion and cell-specific immune response (edit)
SARS-CoV-2 (edit) S1 Subunit of Spike Protein (edit) SARS-CoV-2 altering angiogenesis via NRP1 (edit)
SARS-CoV-2 (edit) S1 Subunit of Spike Protein (edit) Soluble ACE2-mediated cell entry of SARS-CoV-2 (edit)
SARS-CoV-2 (edit) S2 Subunit of Spike Protein (edit) Mitochondrial immune response to SARS-CoV-2 (edit)
SARS-CoV-2 (edit) S2 Subunit of Spike Protein (edit) SARS-CoV-2 altering angiogenesis via NRP1 (edit)
SARS-CoV-2 (edit) S2 Subunit of Spike Protein (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) Non-structural protein 10 [SARS CoV-2] (edit) A draft map of SARS-COV-2 signaling pathway map (edit)
SARS-CoV-2 (edit) Non-structural protein 10 [SARS CoV-2] (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) Non-structural protein 10 [SARS CoV-2] (edit) Pathogenesis of SARS-CoV-2 mediated by nsp9-nsp10 complex (edit)
SARS-CoV-2 (edit) non-structural protein 15 [SARS-CoV-2] (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) non-structural protein 15 [SARS-CoV-2] (edit) Endoplasmic reticulum stress response in coronavirus infection (edit)
SARS-CoV-2 (edit) Papain-like proteinase [SARS-CoV-2] (edit) Antiviral and anti-inflammatory effects of Nrf2 on SARS-CoV-2 pathway (edit)
SARS-CoV-2 (edit) Papain-like proteinase [SARS-CoV-2] (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) non-structural protein 5 [SARS-CoV-2] (edit) Antiviral and anti-inflammatory effects of Nrf2 on SARS-CoV-2 pathway (edit)
SARS-CoV-2 (edit) non-structural protein 5 [SARS-CoV-2] (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) surface glycoprotein (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) ORF1a polyprotein;ORF1ab polyprotein (edit) Antiviral and anti-inflammatory effects of Nrf2 on SARS-CoV-2 pathway (edit)
SARS-CoV-2 (edit) ORF1a polyprotein;ORF1ab polyprotein (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) ORF3a protein-encoding gene (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) envelope protein (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) membrane glycoprotein (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) ORF6 protein (edit) SARS-CoV-2 B.1.1.7 variant antagonises innate immune activation (edit)
SARS-CoV-2 (edit) ORF6 protein (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) ORF7a protein (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) ORF7b (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) ORF8 protein (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) nucleocapsid phosphoprotein (edit) SARS-CoV-2 B.1.1.7 variant antagonises innate immune activation (edit)
SARS-CoV-2 (edit) nucleocapsid phosphoprotein (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) ORF10 protein (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) non-structural protein 6 [SARS-CoV-2] (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) Protein 7a [SARS-CoV-2] (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) ORF8 protein [SARS-CoV-2] (edit) Antiviral and anti-inflammatory effects of Nrf2 on SARS-CoV-2 pathway (edit)
SARS-CoV-2 (edit) ORF8 protein [SARS-CoV-2] (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) Non-structural protein 2 [SARS CoV-2] (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) ORF6 protein [SARS-CoV-2] (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) ORF10 protein [SARS-CoV-2] (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) ORF10 protein [SARS-CoV-2] (edit) Hijack of Ubiquitination by SARS-CoV-2 (edit)
SARS-CoV-2 (edit) Non-structural protein 9 [SARS-CoV-2] (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) Non-structural protein 9 [SARS-CoV-2] (edit) Pathogenesis of SARS-CoV-2 mediated by nsp9-nsp10 complex (edit)
SARS-CoV-2 (edit) SARS-CoV-2 NSP9 complex (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) SARS-CoV-2 Spike - human ACE2 receptor complex (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) SARS-CoV-2 Spike - human ACE2-SLC6A19 complex (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) SARS-CoV-2 cleaved Spike protein complex (edit) Linoleic acid metabolism affected by coronavirus infection (edit)
SARS-CoV-2 (edit) SARS-CoV-2 cleaved Spike protein complex (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) SARS-CoV-2 main protease complex (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) SARS-CoV-2 NSP10-NSP16 2'-O-methyltransferase complex (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) SARS-CoV-2 NSP3-NSP4-NSP6 complex (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) Host translation inhibitor nsp1 [SARS-CoV-2] (edit) nsp1 from SARS-CoV-2 inhibits translation initiation in the host cell (edit)
SARS-CoV-2 (edit) Host translation inhibitor nsp1 [SARS-CoV-2] (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) Non-structural protein 4 [SARS-CoV-2] (edit) SARS-CoV-2 and COVID-19 pathway (edit)
SARS-CoV-2 (edit) Non-structural protein 7 [SARS-CoV-2] (edit) Antiviral and anti-inflammatory effects of Nrf2 on SARS-CoV-2 pathway (edit)
SARS-CoV-2 (edit) Non-structural protein 7 [SARS-CoV-2] (edit) SARS-CoV-2 and COVID-19 pathway (edit)

References

  1. Slenter DN, Kutmon M, Hanspers K, Hanspers K, Riutta A, Windsor J, et al. WikiPathways: a multifaceted pathway database bridging metabolomics to other omics research. NAR. 2018 Jan 4;46(D1):D661–7. doi:10.1093/NAR/GKX1064 (Scholia)
  2. Croft D, Mundo AF, Haw R, Milacic M, Weiser J, Wu G, et al. The Reactome pathway knowledgebase. NAR. 2014 Jan;42(Database issue):D472-7. doi:10.1093/NAR/GKT1102 (Scholia)